Journal: PLoS ONE

Article Title: Tomographic optical imaging of cortical responses after crossing nerve transfer in mice

doi: 10.1371/journal.pone.0193017

Figure Lengend Snippet: (A) Original tomographic images in the right S1 (upper panels) and pseudo-color images of fluorescence responses elicited by vibratory stimulation at 50 Hz for 0.5 s applied to the left forepaw (lower panels). The numbers in the upper panels represent the depth (μm) from the cortical surface. The two arrows in the upper panels show the position of an artery that is visible in the leftmost panel but not in the rightmost panel. The circle in the leftmost lower panel shows the circular window in which response amplitudes were measured in ΔF/F 0 . (B) Time courses of the fluorescence responses measured at 50, 200, 400 and 800 μm deep from the cortical surface using a macroconfocal microscope. Data in (A) and (B) were obtained from the same mouse. (C) Amplitudes of fluorescence responses measured at each depth.

Article Snippet: For imaging, we used a macroconfocal microscope (D-Eclipse C1 system, Nikon, Tokyo, Japan) with an objective lens (AZ Plan Fluor, magnification: 5.0×, numerical aperture: 0.5, working distance: 15 mm), which was combined with a zoom magnification of 1.6×.

Techniques: Fluorescence, Microscopy

Journal: PLoS ONE

Article Title: Tomographic optical imaging of cortical responses after crossing nerve transfer in mice

doi: 10.1371/journal.pone.0193017

Figure Lengend Snippet: (A) Original tomographic images in the right S1 (upper panels) and pseudocolor images of fluorescence responses elicited by vibratory stimulation at 50 Hz for 0.5 s applied to the left forepaw (lower panels). (B) Time courses of the fluorescence responses measured at 50, 200, 400 and 800 μm deep from the cortical surface using a macroconfocal microscope. Data in (A) and (B) were obtained from the same mouse. (C) Amplitudes of fluorescence responses measured at each depth.

Article Snippet: For imaging, we used a macroconfocal microscope (D-Eclipse C1 system, Nikon, Tokyo, Japan) with an objective lens (AZ Plan Fluor, magnification: 5.0×, numerical aperture: 0.5, working distance: 15 mm), which was combined with a zoom magnification of 1.6×.

Techniques: Fluorescence, Microscopy

Journal: PLoS ONE

Article Title: Tomographic optical imaging of cortical responses after crossing nerve transfer in mice

doi: 10.1371/journal.pone.0193017

Figure Lengend Snippet: (A) Time courses of the fluorescence responses measured at 50, 200, 400 and 800 μm deep from the cortical surface using a macroconfocal microscope. Statistical differences were evaluated regarding the peak amplitude and peak latency between the untreated and operated mice. (B) Relative response amplitudes at 200 μm normalized to those at 400 μm. This ratio is smaller than 1.0 in the untreated mice, while it was larger than 1.0 in the operated mice. (C) Schematic drawing of the neural circuits in the operated mice.

Article Snippet: For imaging, we used a macroconfocal microscope (D-Eclipse C1 system, Nikon, Tokyo, Japan) with an objective lens (AZ Plan Fluor, magnification: 5.0×, numerical aperture: 0.5, working distance: 15 mm), which was combined with a zoom magnification of 1.6×.

Techniques: Fluorescence, Microscopy